In which blistering disease does indirect immunofluorescence (IIF) on rat oesophagus substrate give false-positive results, making monkey oesophagus the preferred substrate?

• A Bullous pemphigoid
• B Epidermolysis bullosa acquisita
• C Pemphigus vulgaris
• D Pemphigus foliaceus ✓

Explanation

In pemphigus foliaceus, IIF on rat oesophagus gives false-positive results due to cross-reaction with a pemphigus-foliaceus-like antigen in rodent tissue. Monkey oesophagus or human skin are the preferred substrates for accurate IIF in pemphigus foliaceus. This distinction from pemphigus vulgaris (where both substrates are usable) is a tested laboratory detail.

Reference: Neena Khanna Illustrated Synopsis of Dermatology & STD, 6th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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