Pathology · Hepatobiliary and Pancreatic Pathology

A 55-year-old alcoholic develops jaundice, coagulopathy, and encephalopathy over 4 weeks. Liver biopsy shows zone 3 (pericentral) necrosis, Mallory-Denk bodies (intracytoplasmic eosinophilic aggregates), neutrophilic infiltration, and ballooning degeneration. The molecular composition of Mallory-Denk bodies includes:

  • A Amyloid fibrils of misfolded serum albumin
  • B Glycogen-laden smooth ER proliferation
  • C Ubiquitinated, aggregated keratins 8 and 18 with p62/sequestosome-1 and heat shock proteins
  • D Hemosiderin granules from iron overload
Correct answer: C. Ubiquitinated, aggregated keratins 8 and 18 with p62/sequestosome-1 and heat shock proteins

Explanation

Mallory-Denk bodies (formerly Mallory hyaline) are intracytoplasmic inclusions consisting of aggregated intermediate filaments — specifically ubiquitin-labeled, misfolded keratins 8 and 18 — along with p62/sequestosome-1 (an autophagy adapter), ubiquitin-binding proteins, and heat shock proteins HSP70/HSP90. They represent failed proteolytic clearance of damaged cytokeratin. They occur in alcoholic hepatitis, NASH, primary biliary cholangitis, Wilson disease, and other hepatocellular injuries. Mallory-Denk bodies are not specific to alcohol but are very characteristic of alcoholic hepatitis when accompanied by neutrophilic satellitosis.

Reference: Robbins & Cotran Pathologic Basis of Disease, 10th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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