Pathology · Hematological Malignancies (Leukemias, Lymphomas, Myeloma)

A 35-year-old woman presents with mediastinal mass, fever, and night sweats. Biopsy shows large lacunar cells and rare Reed-Sternberg cells in a fibrous background. Immunohistochemistry: CD15+, CD30+, CD45-, PAX5 dim+, EBV-. The translocation most commonly seen in this specific Hodgkin lymphoma subtype is:

  • A t(14;18) involving BCL2 and IGH loci
  • B t(2;5) involving ALK and NPM1
  • C t(11;14) involving CCND1 and IGH
  • D Amplification of chromosome 9p24.1 involving JAK2, PDL1 (CD274), and PDL2
Correct answer: D. Amplification of chromosome 9p24.1 involving JAK2, PDL1 (CD274), and PDL2

Explanation

Nodular sclerosis classical Hodgkin lymphoma (NSCHL) — characterized by lacunar cells, fibrous bands, and mediastinal involvement in young women — frequently shows amplification of 9p24.1, a locus containing JAK2, CD274 (PDL1), and CD273 (PDL2). This amplification activates JAK-STAT6 signaling via JAK2 and upregulates PDL1/PDL2 expression, which mediates immune evasion by suppressing cytotoxic T-cells in the tumor microenvironment. This finding is the molecular basis for the exceptional response of CHL to pembrolizumab (anti-PD1). t(14;18) is follicular lymphoma; t(2;5) is ALCL; t(11;14) is mantle cell lymphoma.

Reference: Robbins & Cotran Pathologic Basis of Disease, 10th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

Written and medically reviewed by the StethoPrep medical team.

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