In the WHO classification of poliomyelitis, Acute Flaccid Paralysis (AFP) surveillance includes the collection of two stool samples. The recommended interval between the two stool samples and the ideal time for collection is:
- A 24 hours apart, within 14 days of onset of paralysis
- B 72 hours apart, within 60 days of onset of paralysis
- C 48 hours apart, within 14 days of onset of paralysis ✓
- D 7 days apart, within 28 days of onset of paralysis
Explanation
For AFP surveillance, two stool specimens are collected at least 24 hours apart (optimally 48 hours apart), each at least 8–10 grams, within 14 days of onset of paralysis. Adequate stool sample collection is the gold standard for poliovirus isolation and differentiating wild poliovirus from vaccine-derived poliovirus. Specimens should be maintained in cold chain during transport.
Reference: Park's Textbook of Preventive and Social Medicine, 27th ed.
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Written and medically reviewed by the StethoPrep medical team.