Physiology · Renal Physiology (GFR, Tubular Function, Acid-Base, Concentration)

The Henderson-Hasselbalch equation predicts that for every 1 mEq/L rise in HCO3-, the pH change in the blood is:

  • A 0.015 pH units, assuming PaCO2 does not change
  • B 0.05 pH units, regardless of the starting [HCO3-]
  • C Variable and dependent on the buffering capacity at that particular pH and [HCO3-]
  • D Exactly 0.10 because of the 10:1 buffer relationship
Correct answer: C. Variable and dependent on the buffering capacity at that particular pH and [HCO3-]

Explanation

The Henderson-Hasselbalch equation gives pH = pKa + log([HCO3-]/[H2CO3]). A change in HCO3- by 1 mEq/L at a constant PaCO2 does not produce a fixed pH change — it depends on the logarithmic relationship and the prevailing [HCO3-]. At normal values (HCO3- ~24, PaCO2 ~40), a 1 mEq/L HCO3- change approximates ~0.015 pH units, but this differs at other HCO3- values. The key concept is that the pH-HCO3- relationship is logarithmic and non-linear. Option A gives the correct approximate value at normal range, but Option C correctly identifies the variable/dependent nature as the 'best' physiological answer.

Reference: Guyton & Hall, Textbook of Medical Physiology, 14th ed.

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