Pharmacology · Antibacterial Spectrum (Aminoglycosides, Macrolides, Tetracyclines, Metronidazole)

Metronidazole is selectively toxic to anaerobic organisms and microaerophilic protozoa because:

  • A It binds only the ferredoxin-type electron transport proteins present exclusively in anaerobes
  • B Its nitro group is reductively activated to cytotoxic radical intermediates only in low-oxygen environments by anaerobic/reduced ferredoxin systems
  • C Aerobic bacteria lack the outer membrane porins needed for metronidazole uptake
  • D Metronidazole inhibits aerobic respiration selectively in organisms with complex I of the electron transport chain
Correct answer: B. Its nitro group is reductively activated to cytotoxic radical intermediates only in low-oxygen environments by anaerobic/reduced ferredoxin systems

Explanation

Metronidazole is a prodrug; its nitro group must be reduced to hydroxylamine radical intermediates by low-redox-potential electron carriers (ferredoxin, NADH-nitroreductase, pyruvate-ferredoxin oxidoreductase) found in anaerobes and microaerophilic organisms (Giardia, Trichomonas, Entamoeba). These radicals cause single-strand DNA breaks. In aerobic cells, oxygen instantly re-oxidises any reduced intermediates before they can damage DNA, providing selectivity. The answer A is close but imprecise — metronidazole's selectivity is about the reducing environment, not binding specificity.

Reference: KD Tripathi, Essentials of Medical Pharmacology, 8th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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