Pharmacology · Antibacterial Spectrum (Aminoglycosides, Macrolides, Tetracyclines, Metronidazole)

Tigecycline overcomes tetracycline resistance mediated by Tet(M) and Tet(O) efflux pumps because:

  • A Tigecycline is actively concentrated by bacterial tetracycline efflux pumps rather than expelled
  • B The 9-t-butylglycylamide side chain at ring D sterically prevents recognition by Tet(M)/Tet(O) efflux proteins and ribosomal protection proteins
  • C Tigecycline binds irreversibly to 30S ribosomal RNA, unlike reversible tetracycline binding
  • D Tigecycline is activated by bacterial β-lactamases to a form that bypasses efflux pumps
Correct answer: B. The 9-t-butylglycylamide side chain at ring D sterically prevents recognition by Tet(M)/Tet(O) efflux proteins and ribosomal protection proteins

Explanation

Tigecycline is a glycylcycline derivative with a bulky 9-t-butylglycylamide substituent at position 9 of the tetracycline D ring. This modification sterically prevents recognition by both major tetracycline resistance mechanisms: (1) Tet efflux pumps (Tet(A)-(E) for classical tetracyclines) do not efficiently recognise and export tigecycline; (2) ribosomal protection proteins (Tet(M), Tet(O)) that normally dislodge tetracycline from the ribosome cannot effectively recognise the modified D-ring. Tigecycline retains 5-fold higher affinity for the ribosome than tetracycline.

Reference: KD Tripathi, Essentials of Medical Pharmacology, 8th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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