Ophthalmology · Refractive Surgery and Contact Lenses (LASIK, SMILE, PRK, Keratoconus Management)

Corneal collagen cross-linking (CXL) with riboflavin and UVA light is the treatment of choice for progressive keratoconus. The primary mechanism by which CXL halts progression is:

  • A Increasing corneal anterior curvature to reduce ectasia
  • B Stimulating keratocyte proliferation to regenerate lost stromal collagen
  • C Creating new covalent bonds (pyridinium cross-links) between collagen fibrils, increasing corneal stiffness by 4–5 times
  • D Depositing riboflavin in the stroma to block UV-induced proteolysis
Correct answer: C. Creating new covalent bonds (pyridinium cross-links) between collagen fibrils, increasing corneal stiffness by 4–5 times

Explanation

CXL works by riboflavin (vitamin B2) acting as a photosensitizer that, when activated by 365 nm UVA light, generates reactive oxygen species (singlet oxygen). These reactive species induce covalent cross-links (pyridinium cross-links) between lysine groups of adjacent collagen fibrils in the corneal stroma — measurably increasing stromal stiffness by approximately 4–5 times. This biomechanical strengthening halts ectasia progression. Standard CXL requires epithelial removal (epi-off) for adequate riboflavin penetration; transepithelial (epi-on) CXL uses modified riboflavin formulations but is generally considered less effective.

Reference: Khurana Comprehensive Ophthalmology, 7th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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