Microbiology · Gram-Negative Bacteria (E. coli, Salmonella, Shigella, Vibrio, Klebsiella)

Vibrio cholerae O1 is uniquely identified by its 'string test' positivity. This test demonstrates which property of the organism?

  • A Production of extracellular DNase
  • B Flagellar motility enabling rapid spreading
  • C Mucoid colony formation due to abundant extracellular polysaccharide
  • D Hemagglutination of chicken erythrocytes
Correct answer: C. Mucoid colony formation due to abundant extracellular polysaccharide

Explanation

The string test involves spreading a loopful of the bacterial colony on a slide with 0.5% sodium deoxycholate. V. cholerae O1 (both biotypes) forms a mucoid string when touched with a loop due to its copious capsular polysaccharide. It is a rapid presumptive identification test. Flagellar motility is demonstrated by darting motility in hanging drop preparation.

Reference: Ananthanarayan and Panicker's Textbook of Microbiology, 11th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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