Microbiology · Gram-Negative Bacteria (E. coli, Salmonella, Shigella, Vibrio, Klebsiella)

The El Tor biotype of Vibrio cholerae O1 causing pandemic cholera differs from the Classical biotype in that El Tor:

  • A Produces a more potent cholera toxin and causes more severe clinical disease
  • B Is Voges-Proskauer positive, haemolyses sheep erythrocytes, and is resistant to polymyxin B
  • C Has replaced Classical biotype in all current outbreaks and does not produce cholera toxin
  • D Is serogrouped as O139 Bengal, requiring additional vaccine formulation
Correct answer: B. Is Voges-Proskauer positive, haemolyses sheep erythrocytes, and is resistant to polymyxin B

Explanation

El Tor biotype (responsible for 7th pandemic) differs from Classical biotype in several phenotypic characteristics: El Tor is Voges-Proskauer (VP) positive (acetoin production), haemolyses sheep and goat erythrocytes (El Tor haemolysin), is resistant to polymyxin B (50 µg), and agglutinates chicken erythrocytes (Greig test). Classical biotype is VP negative, non-haemolytic, and polymyxin B-sensitive. Both biotypes belong to O1 serogroup (Ogawa and Inaba subtypes) and produce identical cholera toxin; both cause indistinguishable clinical disease. O139 Bengal is a different serogroup causing epidemics in Asia.

Reference: Ananthanarayan & Paniker's Textbook of Microbiology, 11th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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