Microbiology · General Microbiology (Bacterial Genetics, Culture Media, Stains, Sterilization)

Milk is heated to 72 degrees Celsius for 15 seconds followed by rapid cooling. The main purpose of this holder method of pasteurization is to:

  • A Achieve complete sterility of milk including spores
  • B Reduce the microbial count by exactly one log cycle
  • C Destroy Staphylococcus aureus enterotoxin already present in milk
  • D Kill all vegetative pathogens such as Mycobacterium bovis, Brucella, and Coxiella while preserving milk quality
Correct answer: D. Kill all vegetative pathogens such as Mycobacterium bovis, Brucella, and Coxiella while preserving milk quality

Explanation

Holder pasteurization at 63 degrees Celsius for 30 minutes or flash (HTST) method at 72 degrees Celsius for 15 seconds kills vegetative pathogens including the most heat-resistant milk-borne agents, Coxiella burnetii and Mycobacterium bovis. Pasteurization is not sterilization since spores survive, and preformed toxins like staphylococcal enterotoxin are heat stable and unaffected.

Reference: Robbins and Cotran Pathologic Basis of Disease, 10th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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