Microbiology · General Microbiology (Bacterial Genetics, Culture Media, Stains, Sterilization)

The phenomenon of bacterial transduction involves transfer of bacterial DNA via:

  • A Direct cell-to-cell contact through a pilus (F-pilus)
  • B Uptake of free DNA from the environment by competent bacteria
  • C Conjugative plasmid transfer through cell-to-cell fusion
  • D Bacteriophage carrying bacterial chromosomal or plasmid DNA from donor to recipient
Correct answer: D. Bacteriophage carrying bacterial chromosomal or plasmid DNA from donor to recipient

Explanation

Transduction is horizontal gene transfer mediated by bacteriophages that accidentally package bacterial DNA instead of (or in addition to) their own DNA, then inject it into a new recipient bacterial cell. Generalized transduction packages random chromosomal fragments (occurs during lytic phage cycle); specialized transduction packages genes adjacent to the prophage integration site (only during induction of lysogenic phage). Conjugation requires direct pilus-mediated cell contact. Transformation involves uptake of free DNA by competent cells. Transfer by cell fusion is not a standard bacterial mechanism.

Reference: Ananthanarayan & Paniker's Textbook of Microbiology, 11th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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