Medicine · Liver Disease (Cirrhosis, Hepatitis, Autoimmune, Wilson's, Hemochromatosis)

A 25-year-old man has progressive neurological symptoms (dysarthria, tremor, psychiatric changes), hepatic dysfunction, and Kayser-Fleischer rings on slit-lamp. Serum ceruloplasmin is 8 mg/dL (low) and 24-hour urine copper is 280 µg/day (elevated). Which finding in Wilson's disease is most sensitive for the diagnosis when ceruloplasmin is normal?

  • A KF rings alone are pathognomonic even without low ceruloplasmin
  • B Serum free copper (non-ceruloplasmin-bound) > 50 µg/dL
  • C Liver copper content on biopsy > 250 µg/g dry weight is the most sensitive test
  • D ATP7B gene sequencing showing homozygous H1069Q mutation
Correct answer: C. Liver copper content on biopsy > 250 µg/g dry weight is the most sensitive test

Explanation

Liver biopsy copper quantification (> 250 µg/g dry weight) is the gold standard and most sensitive test for Wilson's disease, particularly when ceruloplasmin is normal (occurs in ~5-15% of Wilson's patients) or in acute liver failure where ceruloplasmin may be falsely elevated as an acute phase reactant. Serum free copper > 50 µg/dL and 24-hour urine copper > 100 µg/day are supportive. KF rings are absent in ~50% of hepatic-predominant Wilson's and are not always pathognomonic (can occur in cholestatic liver diseases). ATP7B mutation analysis is useful but multiple mutations exist and not all are identified.

Reference: Harrison's Principles of Internal Medicine, 21st ed.

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