Chromatin immunoprecipitation (ChIP) is a technique used to study which of the following?
- A Protein-DNA interactions in vivo ✓
- B Alternative RNA splicing patterns
- C Lipid membrane composition
- D Mitochondrial DNA heteroplasmy
Correct answer: A. Protein-DNA interactions in vivo
Explanation
ChIP crosslinks proteins to DNA in living cells, shears the chromatin, and uses specific antibodies to immunoprecipitate the protein of interest along with its bound DNA fragments. The associated DNA is then identified by PCR or sequencing, revealing in vivo protein-DNA interactions such as transcription factor binding sites.
Reference: Molecular Cell Biology, 8th ed.
High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP
Written and medically reviewed by the StethoPrep medical team.