Bisulfite treatment of genomic DNA followed by PCR amplification and sequencing is used to determine which of the following?
- A Chromosomal translocations
- B Gene copy number variations
- C DNA methylation patterns ✓
- D Single nucleotide polymorphisms
Correct answer: C. DNA methylation patterns
Explanation
Bisulfite treatment converts unmethylated cytosine residues to uracil, while 5-methylcytosine remains unchanged. After PCR, uracil is read as thymine, allowing distinction between methylated and unmethylated cytosines. This is the gold standard for detecting DNA methylation at CpG islands.
Reference: Robbins and Cotran Pathologic Basis of Disease, 10th ed.
High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP
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