Which statement correctly distinguishes a cDNA library from a genomic DNA library?

• A A cDNA library contains introns, while a genomic library does not
• B A cDNA library requires restriction enzymes but a genomic library does not
• C A genomic library is constructed using reverse transcriptase
• D A cDNA library represents only genes expressed in a specific tissue at a specific time ✓

Explanation

A cDNA library is synthesized from mRNA using reverse transcriptase, so it represents only expressed genes and lacks introns. A genomic library contains the entire genome including introns and regulatory sequences. Both libraries require restriction enzymes for cloning into vectors.

Reference: Molecular Cell Biology, 8th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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