In Western blotting, after proteins are separated by SDS-PAGE and transferred to a nitrocellulose membrane, how is the target protein specifically detected?
- A Binding of a specific primary antibody followed by an enzyme-conjugated secondary antibody ✓
- B Hybridization with a radiolabeled complementary DNA probe
- C Staining with Coomassie brilliant blue dye
- D Autoradiography after incorporation of 32P-labeled nucleotides
Correct answer: A. Binding of a specific primary antibody followed by an enzyme-conjugated secondary antibody
Explanation
Western blotting detects proteins using specific antibodies. B primary antibody binds the target protein, and an enzyme-conjugated secondary antibody (against the primary) enables detection by chemiluminescence or colorimetric substrate. DNA probes are used for Southern blotting, Coomassie stains total protein, and radiolabeled nucleotides are used in probe synthesis.
Reference: Molecular Biology of the Cell, 6th ed.
High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP
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