A researcher wishes to detect the expression level of a specific mRNA in cardiac muscle tissue. Which blotting technique is most appropriate for this purpose?

• A Southern blotting
• B Southwestern blotting
• C Western blotting
• D Northern blotting ✓

Explanation

Northern blotting detects specific RNA molecules after electrophoretic separation and transfer to a membrane, using a labeled complementary probe. Southern blotting detects DNA, Western blotting detects proteins using antibodies, and Southwestern blotting detects DNA-binding proteins. The naming convention follows the target nucleic acid or protein.

Reference: Robbins and Cotran Pathologic Basis of Disease, 10th ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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