In recombinant DNA technology, after a restriction enzyme cuts a plasmid vector and generates sticky ends that pair with a foreign DNA fragment, which enzyme is required to permanently join the two DNA molecules by forming phosphodiester bonds?
- A DNA polymerase I
- B DNA ligase ✓
- C Reverse transcriptase
- D Restriction endonuclease
Correct answer: B. DNA ligase
Explanation
DNA ligase permanently joins the plasmid vector and foreign DNA by catalyzing the formation of phosphodiester bonds between adjacent nucleotides. DNA polymerase synthesizes new DNA strands. Restriction endonucleases cut DNA, and reverse transcriptase synthesizes DNA from RNA.
Reference: Harper's Illustrated Biochemistry, 32nd ed.
High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP
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