In a Western blot procedure, following the electrophoretic separation of proteins and transfer to a membrane, the target protein is specifically detected using which of the following?
- A A labeled complementary DNA probe
- B A fluorescent dye binding all proteins non-specifically
- C A specific primary antibody ✓
- D A radiolabeled dideoxynucleotide
Correct answer: C. A specific primary antibody
Explanation
Western blotting involves separating proteins via gel electrophoresis and transferring them to a membrane. Detection requires a specific primary antibody that binds the target protein, unlike Southern or Northern blots which use nucleic acid probes to detect DNA or RNA respectively.
Reference: Robbins and Cotran Pathologic Basis of Disease, 10th ed.
High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP
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