A researcher wants to detect the expression level of a specific mRNA in a tissue sample. Which technique uses a labeled nucleic acid probe to hybridize with and detect this RNA molecule?
- A Southern blot
- B Northern blot ✓
- C Western blot
- D Eastern blot
Correct answer: B. Northern blot
Explanation
Northern blot is specifically designed to detect RNA sequences using a labeled nucleic acid probe. Southern blot detects DNA sequences, while Western blot detects proteins using specific antibodies. Eastern blot is not a standard term for primary nucleic acid or protein detection in this context.
Reference: Harper's Illustrated Biochemistry, 32nd ed.
High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP
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