Biochemistry · Lipid Chemistry (Sphingolipids, Eicosanoids, Ketogenesis)

Gaucher disease is the most common lysosomal storage disorder. Glucocerebrosidase (glucosylceramidase) deficiency leads to glucocerebroside accumulation in macrophages. Which cell feature is pathognomonic on bone marrow biopsy?

  • A Sea-blue histiocytes with foamy cytoplasm containing phospholipid
  • B Cherry-red spot on macroscopic examination of the marrow
  • C Gaucher cells — engorged macrophages with crinkled tissue-paper or wrinkled silk cytoplasm from stored glucocerebroside, PAS-positive
  • D Balloon cells with clear vacuolated cytoplasm (Niemann-Pick foam cells)
Correct answer: C. Gaucher cells — engorged macrophages with crinkled tissue-paper or wrinkled silk cytoplasm from stored glucocerebroside, PAS-positive

Explanation

Gaucher cells are macrophages massively engorged with glucocerebroside (glucosylceramide). The stored substrate gives the cytoplasm a characteristic crinkled or wrinkled tissue-paper (crumpled silk) appearance on Wright-Giemsa stain, and cells are strongly PAS-positive. They accumulate in bone marrow, spleen, and liver causing hepatosplenomegaly, bone marrow infiltration, anaemia, thrombocytopenia, and bone disease (Erlenmeyer flask deformity). Sea-blue histiocytes contain ceroid and are seen in NPC type B and Niemann-Pick disease. Cherry-red spot is a fundal finding in GM1/GM2 gangliosidoses and NPC. Foam cells (Niemann-Pick cells) contain sphingomyelin.

Reference: Harper's Illustrated Biochemistry, 32nd ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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