Biochemistry · Free Radicals, Antioxidant Defence and Xenobiotic Metabolism

A patient develops cyanosis unresponsive to oxygen therapy after starting dapsone. The blood sample appears chocolate-brown and does not turn red on exposure to air. The primary enzymatic pathway that reduces methemoglobin (Fe3+) back to functional hemoglobin (Fe2+) in erythrocytes uses which electron donor?

  • A NADPH
  • B NADH
  • C Reduced glutathione
  • D Ascorbate
Correct answer: B. NADH

Explanation

NADH-cytochrome b5 reductase is the primary methemoglobin reductase, accounting for about two-thirds of reduction capacity. The minor NADPH-methemoglobin reductase pathway requires an artificial electron carrier like methylene blue to function. Dapsone oxidizes hemoglobin to methemoglobin, overwhelming the NADH-dependent pathway.

Reference: Harrison's Principles of Internal Medicine, 21st ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

Written and medically reviewed by the StethoPrep medical team.

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