Benzoic acid present in food preservatives is detoxified in the liver before renal excretion. The conjugation reaction involved, and its amino acid donor, are:

• A Acetylation with acetyl-CoA
• B Methylation with S-adenosylmethionine
• C Glycination with glycine, forming hippuric acid ✓
• D Amidation with glutamine

Explanation

Benzoic acid is first activated to benzoyl-CoA by an acyl-CoA synthetase, then conjugated with glycine by glycine N-acyltransferase to form hippuric acid, which is excreted in urine. The classical hippuric acid test used this pathway to assess liver function. Acetylation applies to arylamines like isoniazid and sulfonamides, not carboxylic acids, which rules out that distractor.

Reference: Harper's Illustrated Biochemistry, 32nd ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

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