Biochemistry · Free Radicals, Antioxidant Defence and Xenobiotic Metabolism

During ischemia, ATP is degraded to hypoxanthine. Upon reperfusion, xanthine oxidase catalyzes the conversion of hypoxanthine to xanthine, generating a specific reactive oxygen species. Which molecule is the initial ROS produced in this reaction?

  • A Hydrogen peroxide
  • B Superoxide
  • C Hydroxyl radical
  • D Peroxynitrite
Correct answer: B. Superoxide

Explanation

Xanthine oxidase uses molecular oxygen as an electron acceptor during the oxidation of hypoxanthine to xanthine, producing superoxide (O2•−) as the initial reactive oxygen species. Hydrogen peroxide is subsequently formed by superoxide dismutation. This mechanism is central to reperfusion injury.

Reference: Robbins and Cotran Pathologic Basis of Disease, 10th ed.

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