Biochemistry · Free Radicals, Antioxidant Defence and Xenobiotic Metabolism

Rifampicin and St John's Wort both cause therapeutic failure of concurrently administered drugs (e.g., oral contraceptives, antiretrovirals) by which shared mechanism?

  • A Competitive inhibition of CYP3A4 in the intestinal wall and liver
  • B Transcriptional upregulation of CYP3A4 and P-glycoprotein via activation of the pregnane X receptor (PXR)
  • C Irreversible suicide inhibition of CYP2D6
  • D Inhibition of UGT1A1-mediated glucuronidation in the enterocytes
Correct answer: B. Transcriptional upregulation of CYP3A4 and P-glycoprotein via activation of the pregnane X receptor (PXR)

Explanation

Rifampicin and hyperforin (active constituent of St John's Wort) are potent agonists of the pregnane X receptor (PXR, NR1I2), a nuclear receptor that heterodimerises with RXR and transcriptionally induces CYP3A4, CYP2C9, and the drug efflux transporter P-glycoprotein (MDR1) in hepatocytes and enterocytes. This massively increases first-pass metabolism and efflux of co-administered drugs, reducing their systemic bioavailability. This is enzyme induction, not inhibition; inhibitors (e.g., ketoconazole, grapefruit) cause the opposite effect. CYP2D6 suicide inhibition is the mechanism of drugs like paroxetine and haloperidol.

Reference: Harper's Illustrated Biochemistry, 32nd ed.

High-yield for: NEET PGINI-CETNExTFMGEUSMLEPLABMRCP

Written and medically reviewed by the StethoPrep medical team.

Sponsored

Want to test yourself?

Create a free account for timed mock tests, mistake tracking, and FSRS spaced-repetition revision across 23,000+ MCQs.

Start free → Log in

More Free Radicals, Antioxidant Defence and Xenobiotic Metabolism MCQs

See all Free Radicals, Antioxidant Defence and Xenobiotic Metabolism MCQs →